abstract：:Peroxynitrite and hydroxyl radical are reactive oxidants produced during myocardial reperfusion injury. In various cell types, including macrophages and smooth muscle cells, peroxynitrite and hydrogen peroxide cause DNA single strand breakage, which triggers the activation of the nuclear enzyme poly (ADP-ribose) synth...

journal_title：Journal of molecular and cellular cardiology

authors： Gilad E,Zingarelli B,Salzman AL,Szabó C

更新日期：1997-09-01 00:00:00

abstract：:Numerous studies suggest that in adult hearts myocardial ischemic injury is in part the result of proton stimulation of Na/H exchange which increases intracellular Na (Nai) and thus leads to increases in intracellular Ca concentration ( [Ca]i) due to changes in Na/Ca exchange flux. Corollary to the hypothesis, inhibit...

journal_title：Journal of molecular and cellular cardiology

authors： Liu H,Cala PM,Anderson SE

更新日期：1997-08-01 00:00:00

abstract：:Several investigators have demonstrated that the opioid pathway is involved in tissue preservation during hypoxia or ischemia and that this protection is mediated via the delta (delta)-opioid receptor. Subsequently, we have shown that opioid receptors are involved in ischemic preconditioning (PC) in the rat heart and ...

journal_title：Journal of molecular and cellular cardiology

authors： Schultz JJ,Hsu AK,Gross GJ

更新日期：1997-08-01 00:00:00

abstract：:There is increasing evidence that pathological changes in the myocardium during chronic heart failure (CHF) are partly regulated through the activation of the renin-angiotensin system (RAS), an effect mediated by the angiotensin II type 1 receptor (AT1R). We examined the expression of cardiac AT1R mRNA in normal (atri...

journal_title：Journal of molecular and cellular cardiology

authors： Kaprielian RR,Dupont E,Hafizi S,Poole-Wilson PA,Khaghani A,Yacoub MH,Severs NJ

更新日期：1997-08-01 00:00:00

abstract：:Previous studies have demonstrated that pyruvate dehydrogenase kinase (PDHK) activity in extracts of rat cardiac mitochondria is increased @two-fold by providing a high-fat diet for 28 days. The present study sought to establish the factor(s) that might underlie the response of cardiac PDHK to the provision of a high-...

journal_title：Journal of molecular and cellular cardiology

authors： Sugden MC,Orfali KA,Fryer LG,Holness MJ,Priestman DA

更新日期：1997-07-01 00:00:00

abstract：:Previous work suggests that delayed protection against infarction following ischaemic preconditioning of rabbit myocardium may involve the activation of protein kinase C (PKC). Preconditioning in the presence of chelerythrine, an inhibitor of PKC, abolished the late anti-infarct effect of preconditioning. In the studi...

journal_title：Journal of molecular and cellular cardiology

authors： Baxter GF,Mocanu MM,Yellon DM

更新日期：1997-07-01 00:00:00

abstract：:Cardiac myocytes in rat hearts lose their ability to undergo cytokinesis between day 3 and day 4, resulting in the formation of binucleated myocytes. Failure in the formation of the actin-myosin contractile ring could cause cardiac myocytes to be defective in cytokinesis. Enzymatically isolated cardiac myocytes from 2...

journal_title：Journal of molecular and cellular cardiology

authors： Li F,Wang X,Bunger PC,Gerdes AM

更新日期：1997-06-01 00:00:00

abstract：:The biochemical and physiological effects of GS alpha activation are well known; however, little is known about the anatomical localisation of GS alpha in the myocardium. Knowledge of the localisation might yield insights into G protein function in heart. The utility of immunocytochemistry using immunofluorescent meth...

journal_title：Journal of molecular and cellular cardiology

authors： Muntz KH,Ishikawa Y,Wagner T,Homcy CJ,Vatner SF,Vatner DE

更新日期：1997-06-01 00:00:00

abstract：:Microtubules have been implicated in a number of muscle-specific functions, including sarcomerogenesis and the regulation of heart cell beating rate. Post-translationally modified microtubules (MTs) have been correlated, in other cell types, with MTs of increased stability and possibly distinct functions. This study w...

journal_title：Journal of molecular and cellular cardiology

authors： Webster DR

更新日期：1997-06-01 00:00:00

abstract：:The present study was designed in order to investigate more precisely the role of calcium homeostasis maintenance in protein kinase C (PKC) mediated preconditioning. We used a 15 min pre-incubation period, with 1 mumol/l exogenous norepinephrine (NE) to pharmacologically precondition isolated, superfused rat trabecula...

journal_title：Journal of molecular and cellular cardiology

authors： Musters RJ,van der Meulen ET,van der Laarse WJ,van Hardeveld C

更新日期：1997-05-01 00:00:00

abstract：:Aftercontractions induced by beta-adrenoceptor stimulation in human and guinea-pig cardiomyocytes may be related to changes in action potential duration (APD). We investigated the effects of altering APD during the occurrence of isoproterenol-induced aftercontractions, using the KATP channel openers cromakalim and lem...

journal_title：Journal of molecular and cellular cardiology

authors： Tweedie D,O'Gara P,Harding SE,MacLeod KT

更新日期：1997-05-01 00:00:00

abstract：:This study sought to evaluate the use of tetrazolium salt XTT reduction as an indicator of valvular viability in a cryoprocessed porcine cardiac homograft model. The XTT tetrazolium assays was based on the metabolic reduction of Sodium 3'-[1-(phenylamino-carbonyl)-3,4-Tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfon...

journal_title：Journal of molecular and cellular cardiology

authors： Lu JH,Chiu YT,Sung HW,Hwang B,Chong CK,Chen SP,Mao SJ,Yang PZ,Chang Y

更新日期：1997-04-01 00:00:00

abstract：:This paper examines the quantitative relationship between the expression of myosin heavy chain (MHC) and actin at both the levels of their mRNAs and their proteins. Explanted human left ventricle tissues were obtained from non-diseased (ND) individuals and from dilated cardiomyopathy (DCM) patients with terminally fai...

journal_title：Journal of molecular and cellular cardiology

authors： Coumans JV,Yeoh T,Seeto RK,Keogh A,Brennan K,Gunning P,Hardeman E,dos Remedios CG

更新日期：1997-03-01 00:00:00

abstract：:The protection of ischemic preconditioning (PC) appears to be triggered by activation of receptors which couple to protein kinase C (PKC) during the brief ischemia. Previous experiments, however, suggest that phosphorylation of PKC's substrates is not required for the myocytes to enter the preconditioned state. Becaus...

journal_title：Journal of molecular and cellular cardiology

authors： Yang XM,Sato H,Downey JM,Cohen MV

更新日期：1997-03-01 00:00:00

abstract：:The kinetics of Ca influx by Na/Ca exchange into adult rat heart cells loaded with Na and depleted of ATP were investigated, to further elucidate how ATP regulates exchanger properties in the intact heart cell. We found an eight-fold reduction in Vmax for Ca uptake by ATP depletion, with no significant change in the K...

journal_title：Journal of molecular and cellular cardiology

authors： Haworth RA,Biggs AV

更新日期：1997-02-01 00:00:00

abstract：:Effects of acute and chronic treatments with amiodarone, both in the presence and the absence of exogenous triiodothyronine (T3), on repolarizing outward K+ currents were investigated by patch-clamp technique in cultured newborn rat ventricular cells. Acute exposure to amiodarone dose-dependently inhibited the transie...

journal_title：Journal of molecular and cellular cardiology

authors： Guo W,Kamiya K,Toyama J

更新日期：1997-02-01 00:00:00

abstract：:Hemodynamic and electron spin resonance analyses were used to assess the in vivo and in vitro cardioprotective and antioxidant effects of therapeutically relevant doses of Ginkgo biloba extract (EGb 761) and its terpenoid constituents (ginkgolides A and B, bilobalide) in the rat. Significant anti-ischemic effects, ind...

journal_title：Journal of molecular and cellular cardiology

authors： Pietri S,Maurelli E,Drieu K,Culcasi M

更新日期：1997-02-01 00:00:00

abstract：:Familial hypertrophic cardiomyopathy (HCM) is a primary cardiomyopathy with an autosomal dominant pattern of inheritance. The disease bearing genes for HCM in HCM families have been identified as beta-myosin heavy chain, alpha-tropomyosin, cardiac troponin T (cTnT) and myosin binding protein-C genes. In the present st...

journal_title：Journal of molecular and cellular cardiology

authors： Nakajima-Taniguchi C,Matsui H,Fujio Y,Nagata S,Kishimoto T,Yamauchi-Takihara K

更新日期：1997-02-01 00:00:00

abstract：:Cardiac sarcoplasmic reticulum (SR) sequesters Ca2+ and plays a crucial role in the regulation of intracellular Ca2+. Its functional properties are central to the excitation-contraction (E-C) cycle of cardiac muscle. In this study, we examined the hypothesis that alterations in SR function occur during the development...

journal_title：Journal of molecular and cellular cardiology

authors： Ohkusa T,Hisamatsu Y,Yano M,Kobayashi S,Tatsuno H,Saiki Y,Kohno M,Matsuzaki M

更新日期：1997-01-01 00:00:00

abstract：:In view of considerable gaps in our knowledge of myocardial peroxisomes. the aim of the present study is, on the basis of extensive electron-microscopic investigations, to provide reliable results on the inducibility of a proliferation (increase in the number) of these organelles in rodents' heart by several agents an...

journal_title：Journal of molecular and cellular cardiology

authors： Zipper J

更新日期：1997-01-01 00:00:00

abstract：:Although cardiac failure can develop over time after myocardial infarction, the mechanism responsible for this is still unknown. The change of intracellular Ca2+ transport protein, such as sarcoplasmic reticulum (SR) Ca2+-ATPase (SR-Ca2+), Na+-Ca2+ exchanger (Na+-Ca2+), or cardiac phenotypic modulation of contractile ...

journal_title：Journal of molecular and cellular cardiology

authors： Yoshiyama M,Takeuchi K,Hanatani A,Kim S,Omura T,Toda I,Teragaki M,Akioka K,Iwao H,Yoshikawa J

更新日期：1997-01-01 00:00:00

abstract：:The heart responds to increased haemodynamic load with growth of the ventricles. The rise in ventricle mass is due to increasing mass of the myocytes and proliferation of fibroblasts and smooth muscle cells. The accompanying adaptation and remodelling of the interstitium, e.g. production and composition of the extrace...

journal_title：Journal of molecular and cellular cardiology

authors： Simm A,Nestler M,Hoppe V

更新日期：1997-01-01 00:00:00

abstract：:The aim of the study was to test if pre-ischemic treatment with bradykinin can protect against infarction in an isolated rat heart model of regional ischemia and reperfusion, and if any such protection is dependent upon activation of protein kinase C (PKC) or mediated through the nitric oxide (NO) pathway. We also inv...

journal_title：Journal of molecular and cellular cardiology

authors： Bugge E,Ytrehus K

更新日期：1996-12-01 00:00:00

abstract：:Left ventricular hypertrophy is very prevalent among patients with renal insufficiency. Known hypertrophic factors, such as systemic hypertension, do not adequately account for the prevalence of left ventricular hypertrophy in these patients. Circulating growth factors may stimulate cardiomyocyte growth and contribute...

journal_title：Journal of molecular and cellular cardiology

authors： Yu JZ,Bondy GP,Allard MF,Thompson CR,Levin A,McManus BM

更新日期：1996-12-01 00:00:00

abstract：:We studied peripheral skeletal muscle metabolism in monocrotaline-treated rats. Two distinct groups emerged: a percentage of the animals developed ventricular hypertrophy, with no signs of heart failure (compensated group), whilst others, besides ventricular hypertrophy, developed the syndrome of congestive heart fail...

journal_title：Journal of molecular and cellular cardiology

authors： Bernocchi P,Ceconi C,Pedersini P,Pasini E,Curello S,Ferrari R

更新日期：1996-11-01 00:00:00

abstract：:The authors have previously resolved four forms of cyclic nucleotide phosphodiesterase (PDE) in neonatal rat cultured cardiomyocytes by use of high-performance liquid chromatography (HPLC) and have shown that the response of the cGMP-stimulated PDE to the effector cGMP was markedly reduced as compared to that of the c...

journal_title：Journal of molecular and cellular cardiology

authors： Picq M,Dubois M,Grynberg A,Lagarde M,Prigent AF

更新日期：1996-10-01 00:00:00

abstract：:ADP-ribosylation factors (ARFs) are approximately 20-kDa, guanine nucleotide-binding proteins, initially discovered as stimulators of cholera toxin ADP-ribosyltransferase activity and subsequently shown to participate in vesicular trafficking. Five of the six mammalian ARFs have been identified in human tissues by mol...

journal_title：Journal of molecular and cellular cardiology

authors： Lee CM,Stevens LA,Hsu HC,Tsai SC,Lee YT,Moss J,Vaughan M

更新日期：1996-09-01 00:00:00

abstract：:The authors have shown that stimulation of cardiac alpha 1-adrenoceptors confers immediate cardioprotection in the isolated rat heart against post-ischemic dysfunction, and have recently demonstrated that in vivo treatment of rats with norepinephrine (NE) induces cardiac heat shock protein 72 and myocardial adaptation...

journal_title：Journal of molecular and cellular cardiology

authors： Meng X,Cleveland JC Jr,Rowland RT,Mitchell MB,Brown JM,Banerjee A,Harken AH

更新日期：1996-09-01 00:00:00

abstract：:The myocardial extracellular matrix (ECM) is composed of three important constituents: (1) fibrillar collagen, (2) a basement membrane, and (3) proteoglycans. Structural or compositional changes in these ECM components may affect left ventricular (LV) function as well as influence overall LV geometry. Accordingly, thi...

journal_title：Journal of molecular and cellular cardiology

authors： Spinale FG,Zellner JL,Johnson WS,Eble DM,Munyer PD

更新日期：1996-08-01 00:00:00

abstract：:Modification of gene expression in pulmonary arterial smooth muscle cells (PASMCs) could be a valuable tool for investigating the role of specific gene products in normal and pathological PASMC growth, and a novel potential therapy for pulmonary vascular diseases. To examine the direct role of fosB protein in PASMC gr...

journal_title：Journal of molecular and cellular cardiology

authors： Lu CY,Giordano FJ,Rogers KC,Rothman A

更新日期：1996-08-01 00:00:00

abstract：:Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), which act as cardiac hormones, are produced mainly by the atrium and ventricle, respectively, and are involved in body fluid homeostasis and blood pressure control. The ANP and BNP gene expressions are markedly augmented in ventricles of patients wi...

journal_title：Journal of molecular and cellular cardiology

authors： Tamura N,Ogawa Y,Yasoda A,Itoh H,Saito Y,Nakao K

更新日期：1996-08-01 00:00:00

abstract：:This study addressed the question whether the molecular mass of proteins influences their release from isolated rat neonatal cardiomyocytes subjected to simulated ischemia (SI) or metabolic inhibition (MI). During these interventions cellular ATP content and the relative releases of several proteins, ranging in molecu...

journal_title：Journal of molecular and cellular cardiology

authors： Van Nieuwenhoven FA,Musters RJ,Post JA,Verkleij AJ,Van der Vusse GJ,Glatz JF

更新日期：1996-07-01 00:00:00

abstract：:Changes in the capacities of ATP-synthesizing reactions were analysed in residual non-infarcted myocardium following myocardial infarction. Rats were subjected to left coronary artery ligation (MI; n = 11) or to sham operation (sham; n = 18). Two months later, hearts were excised, rinsed and buffer-perfused isovolumic...

journal_title：Journal of molecular and cellular cardiology

authors： Laser A,Ingwall JS,Tian R,Reis I,Hu K,Gaudron P,Ertl G,Neubauer S

更新日期：1996-07-01 00:00:00

abstract：:Ventricular trabeculae from adult and newborn ferret heart were chemically skinned by Triton X-100 which disrupts all cellular membranes. In newborn preparations, maximal Ca(2+)-activated tension was two times smaller than in adult fibres while Ca2+ sensitivity was higher. Caffeine (10 mM) and cyclopiazonic acid (100 ...

journal_title：Journal of molecular and cellular cardiology

authors： Bonnet V,Léoty C

更新日期：1996-06-01 00:00:00

abstract：:The purposes of this study were to: (1) assess myocardial pyruvate dehydrogenase (PDH) activity and substrate exchange under well-perfused and ischemic conditions; (2) determine the metabolic effects of an intra-coronary infusion of the PDH activator, dichloroacetate (DCA); and (3) measure the effects of ischemia and ...

journal_title：Journal of molecular and cellular cardiology

authors： Stanley WC,Hernandez LA,Spires D,Bringas J,Wallace S,McCormack JG

更新日期：1996-05-01 00:00:00

abstract：:We previously reported the induction of calcium-dependent calcium release and depolarization of membrane potential of cardiac mitochondria from rats treated chronically (13 weeks) with doxorubicin. The fact that this was inhibited by cyclosporine A and ruthenium red suggests induction of the mitochondrial permeability...

journal_title：Journal of molecular and cellular cardiology

authors： Solem LE,Heller LJ,Wallace KB

更新日期：1996-05-01 00:00:00

abstract：:We examined metabolic effects of "supply" and "demand" ischemia in immature and mature rabbit hearts. Moderate supply ischemia was produced by a 50% reduction in coronary flow (to approximately 5.0 ml min-1 g-1 giving a 50-55% rise in O2 extraction and a 35% drop in O2 supply/demand). Demand ischemia was produced by s...

journal_title：Journal of molecular and cellular cardiology

authors： Matherne GP,Ely SW,Headrick JP

更新日期：1996-05-01 00:00:00

abstract：:In essential hypertension it has been hypothesized that there is a generalized derangement in cellular Ca2+ homeostasis. The aim of this study was to assess whether there is functional evidence for increased vulnerability to Ca overload in cardiomyocytes derived from age-matched (11-week) normotensive Wistar-Kyoto (WK...

journal_title：Journal of molecular and cellular cardiology

authors： Delbridge LM,Connell PJ,Morgan TO,Harris PJ

更新日期：1996-04-01 00:00:00

abstract：:Stress-induced regulation of the 72 kD heat shock protein (HSP 72), the major stress inducible protein in mammalian cells, is mediated by the activation and binding of a heat shock transcription factor (HSF) to a specific sequence in the 5' region of the promoter termed the heat shock element (HSE). In agreement with ...

journal_title：Journal of molecular and cellular cardiology

authors： Locke M,Tanguay RM,Ianuzzo CD

更新日期：1996-03-01 00:00:00

abstract：:Myocardial perfusion measurement with colored microspheres may become an alternative for radioactive microsphere techniques. We use and validate a spectrophotometric method that has been previously established for large animals in the isolated perfused rat heart. The perfusion system was adapted for use in a NMR micro...

journal_title：Journal of molecular and cellular cardiology

authors： Hiller KH,Adami P,Voll S,Roder F,Kowallik P,Bauer WR,Haase A,Ertl G

更新日期：1996-03-01 00:00:00